Epigenetic services

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Epigenetic services

In the last decade one of the biggest break-through is the chromatin immunoprecipitation and its different modifications (Kuo, M.H. & Allis, C.D,1999 Methods 19, 425-433). With the help of this technique the connection between protein-DNA can be studied inside the cells. Significance of ChIP (Chromatin immunoprecipitation) technology is the possibility of studying the interactions between protein and DNA in native and unmodified state of cell.

If often happens that for each single study we need examine only a well-defined question, and this experience is not available in every lab. In this case we are able to help you as the first Hungarian epigenetic service provider company.

Services:

	In case of gene centered studies we are ready to examine the existing connections between protein-DNA in a defined gene section. Initial sample is the cell line or primary cell culture. We can examine the presence of a defined protein in a specified gene section. Endpoint of study is QPCR, i.e. real time quantitative PCR measurement. In typical ChIP experiment is required a so-called ChIP grade Antibody and the existence of a proven positive control genomic binding region. IgG control is used as negative control.
	Whole genome approach: The break-through of last two year is ChIP Seq, i.e. chromatin immunoprecipitation product sequenced by high-throughput genome sequencer. In this approach we prepare DNA – which is produced by customer or our lab – preparation, sequencing and analysis of primary data.
	MEDIP: Methylated regions are selected by antibody from whole genomic DNA. This fractionated DNA is examined by QPCR on localized genomic regions or by sequencing on whole genome. Great advantage of methodology that the isolation is possible from DNA, which means the previously established DNA banks can be examined.